Generator

Part:BBa_K5319687

Designed by: Zhang Liting   Group: iGEM24_ZJU-China   (2024-10-01)


VioC expression circuit

The device consists of a C4-HSL specific promoter, PRhlR(BBa_J61051), and a downstream VioC(BBa_K5319677). The C4-HSL binds to its specific receptor RhlR(BBa_C0171) and induces the specific promoter PRhlR(BBa_J61051)to express the downstream VioC, thus dye the cloth green. With C4-HSL, we can precisely control the coloring effect of the pigment on the fabric.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1965
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1965
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 786
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1965
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1965
    Illegal NgoMIV site found at 77
    Illegal NgoMIV site found at 618
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2633


[edit]
Categories
Parameters
None